We narrowed to 16,603 results for: pho;

1. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

   Plasmid

   #166853

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641G, I645G

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

   Plasmid

   #91806

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Residues A1529-G1534 replaced with a PreScission …

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

   Plasmid

   #91807

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   PreScission Protease site (LEVLFQGP) inserted bet…

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUS_LC_charge_RtoK

   Plasmid

   #139127

   Purpose

   express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Mutation

   Add K and D to FUS LC so it has the same charge p…

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ-His6-K2P4.1B-mCherry-StreptagII

   Plasmid

   #158744

   Purpose

   Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-B (KCNK4 Human)
   

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-290 and N-linked glycosylation sites r…

   Promoter

   AOX1

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPC-Flag-SOCS1-Y80D

   Plasmid

   #129516

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Aspartic acid

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC-Flag-SOCS1-Y80F

   Plasmid

   #129517

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Phenylalanine

   Promoter

   cmv

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-Fyn-cGASΔN-HA

    Plasmid

    #130922

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Fyn-ΔNcGAS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the dual …

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

    Plasmid

    #128383

    Purpose

    expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted

    Depositor

    Kalle Gehring

    Article

    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-

    Insert

    UbcH7 (UBE2L3 Human)
    

    Tags

    Bactrocera dorsalis parkin with mutation C463A an…

    Expression

    Bacterial

    Mutation

    mutation C86K in UbcH7 gene

    Promoter

    lac promotor

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3_nmPKA-CαN-mPAGFP

    Plasmid

    #114130

    Purpose

    Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. rd

    Plasmid

    #112912

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Tags

    Glutatione-S-Tranferase and precision protease si…

    Expression

    Bacterial

    Mutation

    PEST sequence near the N-terminal removed. 5′-GCG…

    Promoter

    T7

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIR-192-Reporter

    Plasmid

    #71872

    Purpose

    Mammalian expression vector for the analysis of miR-19α activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-192
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-193a-5p-Reporter

    Plasmid

    #71873

    Purpose

    Mammalian expression vector for the analysis of miR-193a-5p activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-193a-5p
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMIR-31-192-193a-Reporter

    Plasmid

    #71874

    Purpose

    Mammalian expression vector for the analysis of miR-31-19α-193a activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Binding sequence targeted by miR-31, miR192, and miR-193a-5p
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

19. LXSN-Cx43-S279A

    Plasmid

    #60705

    Purpose

    retroviral expression of rat Cx43 S279A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S279A

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA-Cx43-S279/282A

    Plasmid

    #60712

    Purpose

    mammalian expression of rat Cx43 S279/282A mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Mutation

    S279/282A

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only