We narrowed to 6,849 results for: GFP expression plasmids

1. pBFC1207

   Plasmid

   #186694

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_2xBsaI_NT (Guide Stuffer) crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUt-mNF-BACH1

   Plasmid

   #199219

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP::BACH1 (BACH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV D2ir promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG302 22aa SunTag NtDRMcd (noNLS) nog

   Plasmid

   #119554

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

   Plasmid

   #225947

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S3E S17E S19E

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

   Plasmid

   #225948

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S3A S17A S19A

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

   Plasmid

   #225956

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Full-length CKAP4 with additional luminal domain …

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

   Plasmid

   #225939

   Purpose

   Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   STIM1 (STIM1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

   Plasmid

   #225944

   Purpose

   Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Evan Reid, Bart Nieuwenhuis

   Inserts

   ATL1 (ATL1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-syn-synaptoPAC-minWPRE

    Plasmid

    #153085

    Purpose

    Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

    Depositor

    Dietmar Schmitz

    Article

    Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

    Inserts

    bPAC(WT)
    Synaptophysin (Syp Rat)
    

    Use

    AAV

    Tags

    His tag, mScarlet, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDL023_Tub-rb_IgG_HC

    Plasmid

    #193620

    Purpose

    α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Tubulin scFv heavy variable + rabbit constant chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDL024_Tub-rb_IgG_LC

    Plasmid

    #193621

    Purpose

    α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Tubulin scFv light variable + rabbit constant chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. CMV PGK1-3xHA

    Plasmid

    #220913

    Purpose

    Expresses PGK1-3xHA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-3xHA (PGK1 Human)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AAV-CAGGS-mNAGA

    Plasmid

    #196448

    Purpose

    Expresses modified α-N-acetylgalactosaminidase in mammalian cells

    Depositor

    Yuichiro Miyaoka

    Article

    Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

    Insert

    modified alpha-N-acetylgalactosaminidase (NAGA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple Gai3

    Plasmid

    #196050

    Purpose

    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TurboID-CRaf

    Plasmid

    #226168

    Purpose

    Lentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-CRaf (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP-IRES-BlastR and Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge