We narrowed to 14,728 results for: egf

1. pcDNA5-hygro-eGFP-STOP-HEC1

   Plasmid

   #114026

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-STOP-HEC1 (NDC80 Human)
   

   Expression

   Mammalian

   Mutation

   Start codon of Hec1 is mutated to stop codon

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1 Dynamin1 R290A D291A T292A

   Plasmid

   #112103

   Purpose

   Mammalian expression of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R290A D291A T292A

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1 Dynamin1 D406A M407A F409A

   Plasmid

   #112108

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406A M407A F409A

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1 Dynamin1 D406R M407R F409A

   Plasmid

   #112110

   Purpose

   Mammalian expression plasmid of GFP-tagged Dynamin protein.

   Depositor

   Justin Taraska

   Article

   Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

   Insert

   Dynamin1 (DNM1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D406R M407R F409A

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP Kind2 S159/181/666E

   Plasmid

   #105314

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666E

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-Akt2(K14A/R25E)-Halo-PS

   Plasmid

   #86632

   Purpose

   Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A/R25E)-Halo-PS (AKT2 )
   

   Expression

   Mammalian

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

   Plasmid

   #84253

   Purpose

   Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

   Plasmid

   #84252

   Purpose

   Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sp sgCD95-2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pES006 (pTet-qacR 2/pQacA-deGFP)

   Plasmid

   #69035

   Purpose

   reporter with qacR 2 on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

   Promoter

   pQacA and pTet

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pES007 (pTet-qacR 5/pQacA-deGFP)

    Plasmid

    #69036

    Purpose

    reporter with qacR 5 on single plasmid

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Inserts

    qacR (qacR Synthetic)
    deGFP
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    W61Y, E90Q, F102S, M116Q, Y119L, E120Q

    Promoter

    pQacA and pTet

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Enh eGFP Reporter-muKC2-X7

    Plasmid

    #59231

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt5-ex3-5
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Dec. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLV-Enh eGFP Reporter-muKC2-X8

    Plasmid

    #59232

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt5-ex7
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLV-Enh eGFP Reporter-muKC2-X5

    Plasmid

    #59229

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt5 ex1
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLV-Enh eGFP Reporter-muKC2-X4

    Plasmid

    #59228

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt4-ex7-9 and 3' UTR
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLV-Enh eGFP Reporter-muKC2-X6

    Plasmid

    #59230

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt5 ex2
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLV-Enh eGFP Reporter-muKC2-X3

    Plasmid

    #59227

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt4-ex7-9
    

    Use

    Lentiviral

    Promoter

    Mu Hsp68 minimal promoter

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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