We narrowed to 8,830 results for: sgRNA

1. pPN144

   Plasmid

   #91673

   Purpose

   Express sgRNA targeting human TBC1D5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TBC1D5 (TBC1D5 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN145

   Plasmid

   #91674

   Purpose

   Express sgRNA targeting human TBC1D5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TBC1D5 (TBC1D5 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0.122

   Plasmid

   #119629

   Purpose

   Level 0 Part. CDS

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   sgRNA scaffold
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_LIG4_Exon3

   Plasmid

   #225363

   Purpose

   pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

   Depositor

   Sujatha Jagannathan

   Article

   Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

   Insert

   LIG4 (LIG4 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQAT02

   Plasmid

   #223374

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT04

   Plasmid

   #223376

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT06

   Plasmid

   #223378

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p8391 LentiCRISPRv2 Neo sgPTPN14-1

   Plasmid

   #221651

   Purpose

   Expression of sgRNA targeting the locus of human PTPN14

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   sgPTPN14-1 (PTPN14 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p8392 LentiCRISPRv2 Neo sgPTPN14-3

    Plasmid

    #221652

    Purpose

    Expression of sgRNA targeting the locus of human PTPN14

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    sgPTPN14-3 (PTPN14 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRhaCAST

    Plasmid

    #211791

    Purpose

    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Rhamnose-inducible

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210736

    Purpose

    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Merry sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210735

    Purpose

    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Gollum sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-Cas9-sgHPRT1

    Plasmid

    #196713

    Purpose

    CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Insert

    Cas9-T2A-BSD-U6-sgHPRT1
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a/hU6

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NarCas9_AAV

    Plasmid

    #192145

    Purpose

    Expresses NarCas9?and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    NarCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCK411.RR1

    Plasmid

    #192644

    Purpose

    Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    BBa_J23119-RR1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-doraWT_rescue_construct

    Plasmid

    #190608

    Purpose

    Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Tags

    3xHA

    Expression

    Insect

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

    Plasmid

    #174118

    Purpose

    Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

    Depositor

    Stanley Qi

    Article

    Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

    Insert

    sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
    

    Use

    Lentiviral

    Promoter

    mU6 and UbC

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX300A-G+Nanog

    Plasmid

    #140280

    Purpose

    CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Promoter

    CBH

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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