We narrowed to 15,981 results for: LEA

1. pClneoMyc MOB1 T12/35A

   Plasmid

   #47938

   Purpose

   expresses Myc tagged human MOB1 containing T12A and T35A mutations

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T12A and T35A

   Promoter

   CMV

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

   Plasmid

   #166413

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   Protection Of Telomeres 1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB deleted 125 a.a

   Promoter

   ef1-a

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 2xNLS-NLP-cMyc LbaCas12a

   Plasmid

   #182123

   Purpose

   pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   2xNLS-NLP-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

   Plasmid

   #44597

   Depositor

   Susan Semple-Rowland

   Article

   Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

   Insert

   EF1a-GFP:2A:CHER(HA)
   

   Use

   Lentiviral

   Promoter

   human elongation factor -1a (pos 2528-3971)

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADGRL3-CTF-Flag

   Plasmid

   #201219

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCHOP10(dLZ).pCDNA1

    Plasmid

    #21900

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Expression

    Mammalian

    Mutation

    The coding region of the murine CHOP-10 cDNA was …

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWay21-MKP-1 ASA

    Plasmid

    #13479

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1ASA (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Arginine 53 to Alanine, Changed Arginine…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABEpuro-Flag-YAP2

    Plasmid

    #27472

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

16. MDH1-miR-31SM-PGK-GFP-Blast

    Plasmid

    #31516

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

17. p3E-DrCav1

    Plasmid

    #194290

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin1 (cav1.S Frog)
    

    Use

    Multisite gateway entry vector

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shGLDC-DOX

    Plasmid

    #72883

    Purpose

    dox inducible knockdown

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    RNAi hairpin against GLDC
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80056

    Purpose

    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    flk1

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge