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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 6521 - 6540 of 14067 results

  3. t1-400

    Plasmid
    #166577
    Purpose
    For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Publication
    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11
    Insert
    Tln1 Head (1-400) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-400 only
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166577-plasmid-map-sequence-id-330256

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  4. pGBW-m4133843

    Plasmid
    #152781
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 leader protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152781-plasmid-map-sequence-id-320777

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  5. pGBW-m4133659

    Plasmid
    #152704
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152704-plasmid-map-sequence-id-320324

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  7. pGBW-m4046374

    Plasmid
    #149120
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149120-plasmid-map-sequence-id-291573

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  8. pGBW-m4046342

    Plasmid
    #149077
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149077-plasmid-map-sequence-id-298000

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  16. TEV-HRV-3C Proteases-mCherry

    Plasmid
    #243795
    Purpose
    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.
    Depositor
    Cole DeForest
    Publication
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TEV and HRV-3C proteases
    Expression
    Mammalian
    Available since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243795-plasmid-map-sequence-id-486454

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  17. pRSET OCaMP

    Plasmid
    #224932
    Purpose
    Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)
    Depositor
    Alexander Lohman
    Publication
    Aggarwal et al Nat Commun. 2026 Jun 22. doi: 10.1038/s4
    Insert
    OCaMP
    Tags
    6xHis, RSET peptide, and Xpress tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224932-plasmid-map-sequence-id-442661

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  20. pCL1313

    Plasmid
    #217955
    Purpose
    Expression of human H3.1 tagged on its C-terminus with eGFP. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Publication
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217955-plasmid-map-sequence-id-430209

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Showing: 6521 - 6540 of 14067 results