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pBbdCas9B(RBSmut)_arr2

Plasmid

#149579

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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HE302: pMVP (L5-L4) RIP

Plasmid

#121728

Purpose

pMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

RIP

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z19

Plasmid

#124791

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV700

Plasmid

#119017

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCHSU64.1-2.2

Plasmid

#115542

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU38.1-2.2

Plasmid

#115540

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU35.1-2.2

Plasmid

#115536

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU31.1-2.2

Plasmid

#115533

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV609

Plasmid

#119014

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV688

Plasmid

#119015

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV689

Plasmid

#119016

Purpose

Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attP sites

Use

CRISPR; Donor vector for creation of platforms fo…

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCHKU30.1-2.2

Plasmid

#115532

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU28.1-2.2

Plasmid

#111049

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU27.1-2.2

Plasmid

#111048

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP605-1

Plasmid

#99482

Purpose

Myc::TEV::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::TEV::Myc

Expression

Bacterial

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP640-2

Plasmid

#99487

Purpose

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Expression

Bacterial

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode49

Plasmid

#83726

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Publication

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode49

Use

Retroviral

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-6NBC-GFP-PGK-Puro-Barcode42

Plasmid

#83719

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Publication

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode42

Use

Retroviral

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-6NBC-GFP-PGK-Puro-Barcode18

Plasmid

#83695

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Publication

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode18

Use

Retroviral

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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