We narrowed to 15,187 results for: pET

1. WP_010880027.1

   Plasmid

   #194729

   Purpose

   Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

   Depositor

   Fang Li

   Article

   Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

   Insert

   nanoparticle-proteinA and His tag
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRM049 His-TEV-Cas9 triNLS 2C

   Plasmid

   #196245

   Purpose

   Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FGFR1_HUMAN_D0

   Plasmid

   #79719

   Purpose

   This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FGFR1 (FGFR1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pEMS1401

   Plasmid

   #29198

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pEMS1538

   Plasmid

   #29261

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   Oct. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMBP-HsSyx3

   Plasmid

   #179287

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Tags

   His6 + mature maltose binding protein + TEV prote…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-HsSyx-375-792

    Plasmid

    #179291

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Tags

    His6 + mature maltose binding protein + TEV prote…

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGLU_115

    Plasmid

    #225795

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Tn5]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLU_79

    Plasmid

    #225780

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[abr]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLU_97

    Plasmid

    #225810

    Purpose

    RS421 library variant 14067

    Depositor

    Nili Ostrov

    Insert

    14067_[Himar]
    

    Use

    Synthetic Biology

    Mutation

    N/A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK635

    Plasmid

    #72473

    Purpose

    pET28a modified to contain TEV protease fusion tag instead of T7 tag

    Depositor

    T. J. Kappock

    Type

    Empty backbone

    Tags

    His6 and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. RP1B-PP1gamma

    Plasmid

    #51770

    Purpose

    Expresses PP1 gamma residues 7-323

    Depositor

    Wolfgang Peti

    Insert

    pp1 gamma
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 gamma residues 7-323

    Promoter

    T7-lac

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. RP1B-PP1beta

    Plasmid

    #51769

    Purpose

    Expresses PP1 beta residues 6-327

    Depositor

    Wolfgang Peti

    Insert

    pp1 beta
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 beta residues 6-327

    Promoter

    T7-lac

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. U6+27-cxcr4

    Plasmid

    #22498

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

19. U6+27-20

    Plasmid

    #21977

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-20 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

20. U6+27-16

    Plasmid

    #21975

    Depositor

    Phil Sharp

    Article

    Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

    Insert

    U6 miR-16 sponge
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only