We narrowed to 1,489 results for: mKate2
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Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNAQ (GNAQ Human)
ExpressionMammalianMutationNLuc inserted at position F(124)EAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneExpressionYeastPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxKate
Plasmid#229946PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)DepositorInsertluxKate
TagsHis-tagExpressionBacterialPromoteraraBADAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNAI3 (GNAI3 Human)
ExpressionMammalianMutationNLuc inserted at position L(91)KAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNAS (GNAS Human)
ExpressionMammalianMutationNLuc inserted at position D(260)NAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-attB53
Plasmid#183610PurposeRMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertPac
UseRmceAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRC
Plasmid#192855PurposeCloning of promoter of interest in front of mkate2 reporter geneDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterno promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105210PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyExpressionMammalianAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNA13 (GNA13 Human)
ExpressionMammalianMutationNLuc inserted at position F(125)DAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNA12 (GNA12 Human)
ExpressionMammalianMutationNLuc inserted at position A(133)FAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPRC6
Plasmid#198429PurposeCloning of promoter of interest in front of mkate2 reporter geneDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterno promoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-5p
Plasmid#103265PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-5p target (MIR155 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105207PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyExpressionMammalianAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmcePromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-5p
Plasmid#103146PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-5p target
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only