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SyNPL: Synthetic notch pluripotent cell lines to monitor and manipulate cell interactions in vitro and in vivo.
Malaguti M, Migueles RP, Annoh J, Sadurska D, Blin G, Lowell S
Development. 2022 May 26. pii: 275525. doi: 10.1242/dev.200226. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183603pPyCAG-EGFP-TM-IRES-PacMammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.
183604pPyPGK-EGFP-TM-IRES-PacMammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
183605pPyCAG-HA-EGFP-Myc-TM-IRES-PacMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.
183606pPyPGK-HA-EGFP-Myc-TM-IRES-PacMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
183607pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-BleMammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.
183608pPyCAG-tagBFP-3xNLS-IRES-HphMammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.
183609pmROSA26-attP50-Neo-mKate2-3xNLS-attP50Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.
183610attB53-Pac-attB53RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
183611attB53-Pac-TRE-mCherry-attB53RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
183612attB53-Pac-tetO-mCherry-attB53RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
183613attB53-Pac-TRE-3xFlag-Neurog1-attB53RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
183614attB53_SNCB+_attB53RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
183615attB53_SNCB-_attB53RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

Antibodies from Article