We narrowed to 7,372 results for: tet

1. GFP-LiMETER-v1

   Plasmid

   #113933

   Purpose

   Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   LOV2 and Rit polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PM-FRB-CFP

   Plasmid

   #67517

   Purpose

   FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   mTOR (MTOR Human)
   

   Tags

   CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

   Expression

   Mammalian

   Mutation

   FRB domain only (residues 2019–2114)

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcU6_1 sgRNA

   Plasmid

   #92395

   Purpose

   Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.1 promoter and gRNA cloning cassette
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.1

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-uTEV1-CB5

   Plasmid

   #171007

   Purpose

   HiLITR protease with CB5 C-terminal tmd targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-CB5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hGFAP-Pink Flamindo-WPRE-SV40

   Plasmid

   #178790

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

   Insert

   Pink Flamindo
   

   Use

   AAV

   Promoter

   hGFAP

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pscAluYNF1

   Plasmid

   #50934

   Purpose

   Expresses scAluY RNA in mammalian cells.

   Depositor

   Katharina Strub

   Article

   Ivanova et al Nucleic Acids Res. 2015 Mar 11;43(5):287

   Insert

   scAluYNF1
   

   Expression

   Mammalian

   Mutation

   the poly(A) linker and the right arm of the dimer…

   Promoter

   7SL RNA promoter (pol III)

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUASTLOTattB_VHH-GFP4::CD8::mCherry

   Plasmid

   #163917

   Purpose

   Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

   Depositor

   Markus Affolter

   Article

   Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

   Insert

   Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tol2-CAG::Nucbow

   Plasmid

   #158992

   Purpose

   Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Nucbow
   

   Tags

   All FPs are fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Dead Streptavidin-SpyCatcher

   Plasmid

   #59547

   Purpose

   pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Dead-dloop-Streptavidin-SpyCatcher
   

   Tags

   N-terminally shortened SpyCatcher

   Expression

   Bacterial

   Mutation

   PolyAsp in L3/4 loop for ion-exchange

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pNBU2_erm_SIE1

    Plasmid

    #136356

    Purpose

    Selection by Inducible Effectors Test Plasmid

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Mutation

    ATG removed to add signal sequence before gene

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDule-Mb haloTyrRS C6

    Plasmid

    #160377

    Purpose

    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

    Depositor

    Ryan Mehl

    Article

    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

    Insert

    C6 HaloTyrosine tRNA synthatase
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Mammalian

    Mutation

    L270S, Y271L, N311G, C313T

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

    Plasmid

    #175020

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    s57a esterase knock out mutant

    Promoter

    CMV

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CFP-CD82

    Plasmid

    #1818

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    CD82 (CD82 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-NIrD

    Plasmid

    #65010

    Purpose

    NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

    Depositor

    Wensheng Wei

    Article

    Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

    Inserts

    rtTA-MAVS(C)
    2A-linked delta-TK
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Tight-TRE

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. integrin beta6 pcDNA1 neo

    Plasmid

    #13580

    Depositor

    Dean Sheppard

    Article

    Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-uTEV1-NES

    Plasmid

    #171006

    Purpose

    HiLITR protease with C-terminal NNES (Cytoplasmic)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-NES
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEIAV-DIO-TLoop-CHR2YFP

    Plasmid

    #66798

    Purpose

    expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

    Depositor

    Edward Callaway

    Article

    Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

    Insert

    Floxed Chr2-YFP
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CMVTetO7 Promoter

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PM2-FRB-CFP

    Plasmid

    #67518

    Purpose

    FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    mTOR (MTOR Human)
    

    Tags

    CFP, HA, and PM tethering sequence from human Lyn…

    Expression

    Mammalian

    Mutation

    FRB domain only (residues 2019–2114)

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAH-CTX1-rhadCas9-native

    Plasmid

    #129392

    Purpose

    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    S. pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine, Histidine 840 to Alanine

    Promoter

    PrhaBAD

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge