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H_NES 41kDa

Plasmid

#201346

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

Plasmid

#177841

Purpose

Mammalian Expression of Folate Receptor

Depositor

Karsten Melcher

Article

Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

Insert

Folate receptor alpha (FOLR1 Human)

Expression

Mammalian

Mutation

all 11 C mutated to S

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_VP30_MARV

Plasmid

#135472

Purpose

Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

VP30

Expression

Mammalian

Mutation

None

Promoter

AG

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox1

Plasmid

#58344

Purpose

expresses human Nox1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al Science. 2000 Jan 7;287(5450):138-42.

Insert

NADPH oxidase 1 (NOX1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-BLU-VIPR

Plasmid

#220497

Purpose

Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

Depositor

Alexander Espinosa

Article

Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Article

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pMV-LEU2

Plasmid

#65334

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

LEU2

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-G1mCNPRS

Plasmid

#174718

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGM265

Plasmid

#220179

Purpose

Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

constitutively active

Promoter

CMV

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hyg-AtAID:HA (p5339)

Plasmid

#239363

Purpose

pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Arabidopsis Auxin Inducible Degradation domain

Use

Bacterial

Tags

1 x HA epitope

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-APEX2-Venus

Plasmid

#73467

Purpose

Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

APEX2-Venus

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGDP3 apmA

Plasmid

#112881

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

apmA (apmA Staphylococcus aureus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 mSynCAM 2

Plasmid

#115497

Purpose

expression of mouse full-length SynCAM 2

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm2 (Cadm2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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