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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,359 results for: 11

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Showing: 6621 - 6640 of 8359 results

  1. SnapTag-Binder

    Plasmid
    #179142
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Publication
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SspB
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179142-plasmid-map-sequence-id-353405

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  2. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198218-plasmid-map-sequence-id-389373

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  3. CD44U-c011

    Plasmid
    #175454
    Purpose
    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    AviTag-His6
    Expression
    Mammalian
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175454-plasmid-map-sequence-id-353220

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  10. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  11. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid
    #242651
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    ABE8.8 mutations in TadA
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242651-plasmid-map-sequence-id-489040

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  12. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  14. pCMV-tadDE-PcrA M6-UGI

    Plasmid
    #221236
    Purpose
    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.
    Depositor
    Fei Chen
    Publication
    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tadDE-PcrA M6-UGI
    Expression
    Mammalian
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221236-plasmid-map-sequence-id-437117

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Showing: 6621 - 6640 of 8359 results