We narrowed to 7,266 results for: 11

1. pCDNA3 Flag BimL(S58D,DE)

   Plasmid

   #25061

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (S58D,DE) (BCL2L11 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed serine 58 to aspartic acid changed leuci…

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pPICZ:hTRAAK(W262S)

   Plasmid

   #130670

   Purpose

   P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   KCNK4 (KCNK4 Human)
   

   Use

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   N104Q, N108Q, W262S

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. HM3C NaVSp1 pET24b

   Plasmid

   #128088

   Purpose

   Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

   Depositor

   Dan Minor

   Article

   Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

   Insert

   NaVSp1p
   

   Use

   Tags

   3C Cleavage, His Tag, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES:hTRAAK(W262S)

   Plasmid

   #133078

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES:hTRAAK(G124I)

   Plasmid

   #133079

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G124I

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. EasyClone-MarkerFree Vector Set

   Plasmid Kit

   #1000000098

   Purpose

   Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9

   Depositor

   Irina Borodina

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

7. BL21(DE3) ΔserC

   Bacterial Strain

   #197656

   Purpose

   Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Bacterial Resistance

   None

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

8. pTSara

   Plasmid

   #60720

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   N Terminal fragment of T7 RNAP split betwen 179-180
   N Terminal fragment of T7 RNAP split betwen 179-180
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T7 RNAP split between positions 179-180 and wt T7…

   Promoter

   PBAD

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8522-M01-663

   Plasmid

   #225660

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiFUCCI(CA)5

    Plasmid

    #223176

    Purpose

    Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

    Depositor

    Neville Sanjana

    Article

    Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

    Insert

    FUCCI(CA)5
    

    Use

    Lentiviral

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR223-EBNA2

    Plasmid

    #195815

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA2 (EBNA-2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-HA-BACE1

    Plasmid

    #165032

    Purpose

    HA-BACE1

    Depositor

    Juan Bonifacino

    Article

    Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    HA (embedded after propeptide cleavage site)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSOD1-FLAG

    Plasmid

    #182922

    Purpose

    Mammalian expression plasmid for human WT SOD1

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    [Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SpCas9(H840A)-T2A-EGFP

    Plasmid

    #221231

    Purpose

    Express nCas9 nickase (H840A) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(H840A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid

    #222878

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SpCas9(D10A)-T2A-EGFP

    Plasmid

    #221230

    Purpose

    Express nCas9 nickase (D10) with GFP reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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