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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,083 results for: sgRNA

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Showing: 6681 - 6700 of 9083 results

  1. pGY151

    Plasmid
    #249723
    Purpose
    pGY151 is a CRISPR cytosine base editing (CBE) vector with the nickase variant of SpCas9-NG (nCas9-NG) and an sgRNA cassette, enabling targeted C-to-T conversions at flexible NG PAM sites in filamento
    Depositor
    Kyle Pomraning
    Article
    Yuan et al Microb Cell Fact. 2026 Mar 26. doi: 10.1
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    March 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    249723-plasmid-map-sequence-id-502508

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  4. pNB-Rep

    Plasmid
    #238996
    Purpose
    Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Article
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    238996-plasmid-map-sequence-id-495027

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  9. pGMF1-M

    Plasmid
    #242203
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242203-plasmid-map-sequence-id-490170

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  10. pGMF2-M

    Plasmid
    #242204
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242204-plasmid-map-sequence-id-490169

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  11. pGMF1-D

    Plasmid
    #242205
    Purpose
    AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    AtU6-26p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242205-plasmid-map-sequence-id-490168

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  14. pYPQAT02

    Plasmid
    #223374
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223374-plasmid-map-sequence-id-452305

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  15. pYPQAT06

    Plasmid
    #223378
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223378-plasmid-map-sequence-id-454174

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  16. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210734
    Purpose
    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Frodo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    210734-plasmid-map-sequence-id-464595

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  20. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Article
    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Rhamnose-inducible
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

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Showing: 6681 - 6700 of 9083 results