We narrowed to 9,000 results for: sgRNA

Showing: 6681 - 6700 of 9000 results

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pCas13

Plasmid

#82381

Purpose

sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23119

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJK430

Plasmid

#155377

Purpose

PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A2NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP (PX458)

Plasmid

#48138

Purpose

Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRGEB32

Plasmid

#63142

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCRISPRia-v2

Plasmid

#84832

Purpose

CRISPRi/a V2 library parental plasmid

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Inserts

sgRNA
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSIBR-Int4-Cas9-NT

Plasmid

#235166

Purpose

Expresses the SIBR-Int4-Cas9 system and a NT sgRNA

Depositor

Constantinos Patinios

Article

Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

Insert

SIBR-Int4-Cas9

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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PX552

Plasmid

#60958

Purpose

pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Inserts

U6_(SpaI)_sgRNA
Syn_EGFP-KASH

Use

AAV, CRISPR, and Mouse Targeting

Expression

Mammalian

Promoter

Synapsin and U6

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pS548∙CsR

Plasmid

#197859

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Tc resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQAT56

Plasmid

#223428

Purpose

T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pS648∙CsR

Plasmid

#197860

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLP110_AAV Scramble Control

Plasmid

#239419

Purpose

Negative control AAV vector for SaCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble control

Use

AAV

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_2_SpGCas9

Plasmid

#233457

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

Plasmid

#187925

Purpose

sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Insert

hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1

Use

CRISPR and Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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