We narrowed to 29,857 results for: pET

1. pET28a SV40 ST full length

   Plasmid

   #132636

   Purpose

   expresses SV40 ST full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

   Insert

   SV40 small t antigen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-H12TEV-hEIF5B-1~1220(FL)

   Plasmid

   #208636

   Purpose

   for E. coli expression of human eIF5B (ENST00000289371)

   Depositor

   Quanfu Ma

   Insert

   human eIF5B (ENST00000289371) (EIF5B Human)
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Contain SNP rs1376443, protein sequence not chang…

   Promoter

   T7

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a(+)-Histag-Citrine (525)

   Plasmid

   #70718

   Purpose

   Expression vector for Citrine purification

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a(+)_C.jejuni m23 peptidase

   Plasmid

   #83297

   Purpose

   Expresses m23 peptidase from Campylobacter jejuni

   Depositor

   SeWon Suh

   Insert

   ERS445056_01343
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-20

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PKCsub-YPet-NES

   Plasmid

   #138207

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKCsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b GFP-Rab10 Q68LHis

   Plasmid

   #186015

   Purpose

   GFP-Rab10 Q68LHis

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   GFP-Rab10 Q68LHis (RAB10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-6xHis-GFP(+36)-DL4

   Plasmid

   #199168

   Purpose

   used to express a supercharged GFP variant that forms inclusion bodies in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+36)-DL4
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

   Promoter

   T7

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET23-CBD-(-PP)-EGFP-H246,249D

   Plasmid

   #12598

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Type

   Empty backbone

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET28a-ClfB_N2N3-HIS-ybbr

    Plasmid

    #101717

    Purpose

    ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ClfB_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet1-WT-LanCL2-C-Ter-His

    Plasmid

    #154186

    Purpose

    To express LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETNKI-his-3C-LIC-kan

    Plasmid

    #108703

    Purpose

    Expression of an His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET23b-Casp9 C287A-His

    Plasmid

    #11830

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 catalytic mutant (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C287A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

14. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-DEST42-ARL8B-V5-His6

    Plasmid

    #67425

    Purpose

    Expression of hArl8b with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-DEST42-ARF1-V5-His6

    Plasmid

    #67411

    Purpose

    Expression of hArf1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-his-Gluc-iLID

    Plasmid

    #172096

    Purpose

    Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - iLID
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gluc Mutations - M43L, M110L

    Promoter

    T7

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET28-his6-HA3-CLIP

    Plasmid

    #188942

    Purpose

    T7 expression vector for making his6- and HA3-tagged CLIP protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-CLIP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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