Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,153 results for: CHL

Clear keywords
Showing: 3381 - 3390 of 12153 results

  1. HUHe variant click editor (CE1) - pCMV-T7-BDFV-nCas9-EcKlenow (JO1271)

    Plasmid
    #217785
    Purpose
    Variant CE1 construct with BDFV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    BDFV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217785-plasmid-map-sequence-id-432848

    Enlarge

  2. HUHe variant click editor (CE1) - pCMV-T7-CoCV-nCas9-EcKlenow (JO1262)

    Plasmid
    #217786
    Purpose
    Variant CE1 construct with CoCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    CoCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217786-plasmid-map-sequence-id-432849

    Enlarge

  3. HUHe variant click editor (CE1) - pCMV-T7-FiCV-nCas9-EcKlenow (JO1263)

    Plasmid
    #217787
    Purpose
    Variant CE1 construct with FiCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    FiCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217787-plasmid-map-sequence-id-432850

    Enlarge

  4. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid
    #217788
    Purpose
    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217788-plasmid-map-sequence-id-432851

    Enlarge

  5. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid
    #217789
    Purpose
    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217789-plasmid-map-sequence-id-432852

    Enlarge

  6. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

    Enlarge

  8. FUGW-Arl13b-PR-EGFP-TurboID

    Plasmid
    #248194
    Purpose
    Lentiviral expression of truncated Arl13b containing the Proline-Rich domain at the C-terminus (cytosol localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b noncilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain the PR domain
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248194-plasmid-map-sequence-id-494577

    Enlarge

  9. FUGW-Arl13b-N+RVEP+PR-EGFP-TurboID

    Plasmid
    #248193
    Purpose
    Lentiviral expression of truncated Arl13b containing the N-terminal amphipathic helix, cilium targeting RVEP, and the Proline-Rich domain at the C-terminus (cilium localization)
    Depositor
    Xuecai Ge
    Article
    Liu et al Nat Commun. 2024 Apr 25;15(1):3365. doi:
    Insert
    The Arl13b cilium targeting sequence-EGFP-TurboID (Arl13b Mouse)
    Use
    Lentiviral
    Tags
    EGFP and TurboID
    Expression
    Mammalian
    Mutation
    Truncated to only contain N + RVEP + PR domains
    Promoter
    hUBC
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    248193-plasmid-map-sequence-id-494576

    Enlarge

Showing: 3381 - 3390 of 12153 results