We narrowed to 15,483 results for: NOL

1. pILGFPB6

   Plasmid

   #83557

   Purpose

   Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL10 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. const #7-RFP

   Plasmid

   #81081

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23109

   Available since

   Aug. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pconst #6-RFP

   Plasmid

   #81080

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Jay Keasling, Paul Adams

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23117

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ARK1a

   Plasmid

   #81016

   Purpose

   E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

   Depositor

   Taek Soon Lee

   Article

   Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

   Inserts

   MevTo-BBa1002-pTrc-Mkco-PMKco
   PMD-NudB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   LacUV5 and Trc promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSTARR-seq_fly-x16

   Plasmid

   #71505

   Purpose

   flySTARR-seq screening vector with x16 core promoter

   Depositor

   Alexander Stark

   Article

   Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-RpS12-Gateway

   Plasmid

   #71508

   Purpose

   flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

   Depositor

   Alexander Stark

   Article

   Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

   Type

   Empty backbone

   Use

   Flystarr-seq validation vector

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSTARR-seq_fly-eEF1delta

   Plasmid

   #71501

   Purpose

   flySTARR-seq screening vector with eEF1delta core promoter

   Depositor

   Alexander Stark

   Article

   Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 ZFYVE9

   Plasmid

   #67134

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ZFYVE9 (ZFYVE9 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   F1359I

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 TCEB3B

   Plasmid

   #67117

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCEB3B (ELOA2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11+PCNA3-CYP119(T214V)

    Plasmid

    #66137

    Purpose

    Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET11+PCNA3-CYP119(D77R/T214V)

    Plasmid

    #66138

    Purpose

    Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBGSA FPR1 Q258A

    Plasmid

    #66724

    Purpose

    Constitutive expression of FPR1 Q258A in mammalian cells.

    Depositor

    Heini Miettinen

    Article

    Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

    Insert

    FPR1 (haplotype 3) (FPR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q258A

    Promoter

    SR-alpha

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gal4pBS13-Pleum

    Plasmid

    #60332

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ROZA YF

    Plasmid

    #64197

    Purpose

    Fluorescent Reporter for ZAP-70 tyrosine kinase activity-control YF mutant

    Depositor

    Annemarie Lellouch

    Article

    Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

    Insert

    ROZA YF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    tyrosine phosphorylatin site mutated to phenylala…

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only