We narrowed to 59,446 results for: cloning

1. Nsp2-SmuCas9_AAV

   Plasmid

   #192144

   Purpose

   Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2-SmuCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK_Citrine_Nanotag_5_neg_Control

   Plasmid

   #130558

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   negative oligo sequence
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT100.110

   Plasmid

   #122602

   Purpose

   clone CDA1 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   CDA1
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. fADL-1e-HA-linker-p3

   Plasmid

   #139440

   Purpose

   For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

   Insert

   linker with pelB-leader, cloning site, HA-tag and p3
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQTEV-MTPN

   Plasmid

   #34829

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   myotrophin (MTPN Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQTEV-STX10

   Plasmid

   #31572

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   syntaxin 10 (STX10 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM116: CasRx VEGFA presgRNA

   Plasmid

   #166868

   Purpose

   U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   Human VEGFA targeting presgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC-U63-QtgRNA2.1-8R

    Plasmid

    #170514

    Purpose

    gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOG140

    Plasmid

    #105399

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    PKI nuclear export signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJOG141

    Plasmid

    #105400

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    SV40 nuclear localization signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO GFP DNAJB7

    Plasmid

    #19503

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB7 (DNAJB7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-IP3KC

    Plasmid

    #32527

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

    Insert

    IP3KC (ITPKC Human)
    

    Tags

    6xHIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pBT100.136c

    Plasmid

    #122603

    Purpose

    clone FERNY phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    FERNY
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM1761

    Plasmid

    #105381

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promoter AtDMC1 (At3G22880; 3000bp)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQTEV-SESN2

    Plasmid

    #34820

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sestrin 2 (SESN2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEG R3-L4 #BGV194

    Plasmid

    #48954

    Purpose

    For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pQTEV-WDR41

    Plasmid

    #34808

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    WD repeat domain 41 (WDR41 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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