Addgene

We narrowed to 2,354 results for: 683

Showing: 661 - 680 of 2354 results

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pCMV-3*flag-NEXN

Plasmid

#216834

Purpose

expressing Falg tag and the coding sequence of mouse Nexn

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Nexn (mus musculus) (Nexn Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

Plasmid

#131683

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

EF1a and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 321-843 REV1

Plasmid

#201587

Purpose

Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 321-843 of DNA repair prote…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

Plasmid

#201545

Purpose

Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks N-terminal residues 1-79

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

Plasmid

#201546

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 188-228

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

Plasmid

#201548

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 364-409

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L

Plasmid

#146838

Purpose

Insect Expression of DmGW182-PAM26B-F1370A-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-PAM26B-F1370A-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delDUF-M2-dsRNAres_L

Plasmid

#146830

Purpose

Insect Expression of DmGW182-delDUF-M2-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-delDUF-M2-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-E962Q-dsRNAres_L

Plasmid

#146834

Purpose

Insect Expression of DmGW182-E962Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-E962Q-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-F961A-dsRNAres_L

Plasmid

#146835

Purpose

Insect Expression of DmGW182-F961A-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-F961A-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-PAM26B-dsRNAres_L

Plasmid

#146837

Purpose

Insect Expression of DmGW182-PAM26B-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-PAM26B-dsRNAres (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEE073

Plasmid

#176830

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

712

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE074

Plasmid

#176831

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

713

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE076

Plasmid

#176833

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

715

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE078

Plasmid

#176835

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

717

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Centrin2

Plasmid

#136834

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin2 (CETN2 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrin2

Plasmid

#136833

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin2 (CETN2 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrobin

Plasmid

#136830

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrobin (CNTROB Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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gh16

Plasmid

#106700

Purpose

expression of gRNA targeting GALNT16

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT16 (GALNT16 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh234

Plasmid

#106848

Purpose

expression of gRNA targeting DPY19L1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

DPY19L1 (DPY19L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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