We narrowed to 712 results for: bacillus
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Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A-SRDX
Plasmid#129684PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b-SRDX
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz74_Rubisco_CsoS2_Npeptide_fusion
Plasmid#140840PurposeSame as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallizationDepositorInsertForm IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
TagsCsoS2 N-peptide consensus to CbbL C-terminus and …ExpressionBacterialPromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB1
Plasmid#162691PurposeExpresses DabAB1 inorganic carbon transport complexDepositorInsertDabAB1 inorganic carbon transport complex
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2 C539A
Plasmid#133008PurposepFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialMutationC539APromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2 H524A
Plasmid#133007PurposepFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialMutationH524APromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabAB2 D353A
Plasmid#133006PurposepFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)DepositorInsertsdabB2
dabA2
ExpressionBacterialMutationD353APromotertetRAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR
Plasmid#136382PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-VPR
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
TagsHis14 tagExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2
Plasmid#133013PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialPromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCM
Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV
Plasmid#136381PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion proteinDepositorInsertAaCas12b (D570A)-TV-T2A-MCP-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 D353A
Plasmid#133016PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationD353APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2 C351A
Plasmid#133014PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
Tags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationC351APromotert7Available SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
enDR3-TEV-mEGFP-NLS (pRS1451)
Plasmid#240546PurposeExpression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.DepositorInsertenDR3
ExpressionMammalianMutationL52MAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
nb-enDR3-TEV-mEGFP-NLS (pRS1458)
Plasmid#240547PurposeExpression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.DepositorInsertnb-enDR3
ExpressionMammalianMutationS48A, K50AAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only