We narrowed to 2,798 results for: fas

1. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. VCP(A232E)-EGFP

   Plasmid

   #23973

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   A232E

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-DivisionRecorder

   Plasmid

   #179446

   Purpose

   Retroviral expression vector containing the DivisionRecorder cassette

   Depositor

   Ton Schumacher

   Article

   Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

   Insert

   GFP-P2A-511-CrePI-511
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-hFADD-FLAG

   Plasmid

   #31814

   Depositor

   Astar Winoto

   Article

   Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

   Insert

   FADD (FADD Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNATM3.1/V5-His TOPO-PKCi-K274W

   Plasmid

   #55690

   Purpose

   expresses dominant negative human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E and K274W

   Promoter

   CMV

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNATM3.1/V5-His TOPO-PKCi-A120E

   Plasmid

   #55687

   Purpose

   expresses constitutively active human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E

   Promoter

   CMV

   Available Since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

   Plasmid

   #183254

   Purpose

   Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

   Depositor

   Alexander McLellan

   Article

   Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

   Insert

   Tissue factor (F3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR.sgKras.9

    Plasmid

    #91894

    Purpose

    sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

    Depositor

    Wen Xue

    Article

    Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    Kras sgRNA
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR1162

    Plasmid

    #111098

    Purpose

    Expresses FANCA_-_89883048.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJC-ase0.8kb-TALE3-VP64-P10

    Plasmid

    #104610

    Purpose

    Expresses TALE3 under the control of a 0.8kb ase enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    0.8kb ase enhancer element (ase Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR1161

    Plasmid

    #111097

    Purpose

    Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCA gRNA (FANCA Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET PI-SceI C-His

    Plasmid

    #195610

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag

    Depositor

    Frederick Gimble

    Article

    He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

    Insert

    PI-SceI
    

    Expression

    Bacterial

    Mutation

    T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…

    Promoter

    T7

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-Q22YU3 del C3-2xStrep

    Plasmid

    #170316

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 C-terminal truncation (Delta-C3)
    

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Deletion of C-terminal C3-finger

    Promoter

    polyhedrin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDDGFP-Leu2d-GgKDELR2 R159K

    Plasmid

    #123626

    Purpose

    Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Lys

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDDGFP-Leu2d-GgKDELR2 H12A

    Plasmid

    #123619

    Purpose

    Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    His12Ala

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDDGFP-Leu2d-GgKDELR2 R47A

    Plasmid

    #123620

    Purpose

    Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Ala

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDDGFP-Leu2d-GgKDELR2 R47K

    Plasmid

    #123621

    Purpose

    Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Lys

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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