...effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods....effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods) can be cloned for expression as...amino-terminus. Additional details about the REAL, REAL-Fast, and FLASH assembly methods can be found on the ...construction of TALE repeat arrays by REAL or REAL-Fast assembly or names of plasmids required for FLASH...

...Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...Cas9 nucleases. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem...Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino...Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F....genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

...superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 GADD45A GADD45B...

... Restriction Cloning and Fast Cloning Requirement Restriction Cloning Fast Cloning Choose Restriction... Transformation + + The FastCloning process in brief In FastCloning, you PCR both the vector and ...and ligated in vivo. Detailed FastCloning procedure To start the FastCloning process, the insert and vector...most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning technique that...understanding of the step by step procedure of FastCloning, here are some tips for high efficiency cloning...of the colonies are correct. Good luck with this fast and easy technique! Many thanks to our guest blogger...PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...

...effects can mask weaker ones. Thus, the need for a faster and higher-throughput in vitro approach. Using ...constructed as well. They called this library the CTD2 FASMIC Plasmid Collection for “Functional Annotation of...

...high levels of correct assemblies) translate to faster assembly workflows that require less screening ...

...Nature . 550(7676):407-410. PMID: 28931002 Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...CRISPR-Cas9. Cell . 167(7):1829-1838. PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

.... https://doi.org/10.1038/nmeth.3993  Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...doi.org/10.1038/s41586-019-0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani.... https://doi.org/10.1016/j.cell.2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

...Thakore PI, Moreb EA, Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. ...Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers...25692716. PubMed Central PMCID: PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ...

...editing using Staphylococcus aureus Cas9. 2015. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... guide RNA and target DNA. 2014. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani...nucleases. 2013. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem...engineering using the CRISPR-Cas9 system. 2013. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F....engineering using CRISPR/Cas systems. 2013. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...

...such as 2-fluoroadenine (2-FA), organisms with functional APT will convert 2-FA to cytotoxic nucleotides...nucleotides, resulting in death. In the presence of 2-FA, potent gene silencing of APT is required for survival...Darren Gilmour at the University of Zurich. He is fascinated by how organisms exquisitely and robustly self-organize...

...editing using Staphylococcus aureus Cas9. 2015. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ... guide RNA and target DNA. 2014. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani...nucleases. 2013. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem...engineering using the CRISPR-Cas9 system. 2013. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F....engineering using CRISPR/Cas systems. 2013. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...

...doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-FA (2020) Imposter syndrome threatens diversity. Science.... https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace uncertainty. Science 351:994–994 ....

....   References and resources References Al-Allaf FA, Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet... containing the recombined product tend to grow faster, so we recommend picking the smaller colonies which...

...AR.  Nature. 2013 Jan; 493, 429–432. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X,...

... https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino...breaks in tens of minutes – an order of magnitude faster than HDR. NHEJ is consequently the principle means...

...complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani...

...engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F....

...Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013...