We narrowed to 7,650 results for: ski

1. pA-01

   Plasmid

   #166923

   Purpose

   LacI expression cassette (A)

   Depositor

   Thomas Gorochowski

   Article

   Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

   Insert

   LacI expression cassette - Block A- Flanked by BsaI sites
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAH1

   Plasmid

   #41825

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Expression

   Bacterial and Yeast

   Promoter

   T7, TEF

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U6-U57C

   Plasmid

   #169928

   Purpose

   WT U6 with a U57C mutation

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   U6 U57C
   

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SG-3'REL

   Plasmid

   #27257

   Depositor

   Thomas Gilmore

   Article

   Starczynowski et al Oncogene. 2003 Oct 9. 22(44):6928-36.

   Insert

   REL (REL Human)
   

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Contains aa 278-587 of REL

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. Vh-HA-3xDHFR

   Plasmid

   #20219

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xDHFR (folA E. coli)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. wt Cus2p

   Plasmid

   #73046

   Purpose

   Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 Dec 2.

   Insert

   CUS2p (CUS2 Budding Yeast)
   

   Tags

   6x His tag fused to TEV cleavage site

   Expression

   Bacterial and Yeast

   Promoter

   T7

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS413 Prp5 WT

   Plasmid

   #87599

   Purpose

   Encodes Prp5 WT for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   PRP5 (PRP5 )
   

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vh-HA-2xDHFR

   Plasmid

   #20218

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xDHFR (folA E. coli)
   

   Tags

   HA and Vh

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pUC57-Homology Arms-BRD4-HaloTag

   Plasmid

   #228575

   Purpose

   CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   Homology Arms-BRD4-HaloTag (BRD4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-U6-BRD4-HaloTag-Guide-hspCas9

    Plasmid

    #228576

    Purpose

    Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

    Depositor

    Marcin Imielinski

    Insert

    BRD4 gRNA (BRD4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBetaActin-dCer3-C2-dCer3

    Plasmid

    #191328

    Purpose

    Expresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membrane

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    dCer3-C2-dCer3
    

    Expression

    Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rp51a Hyperstabilized 5'SS

    Plasmid

    #100988

    Purpose

    pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    5'ss mut ATG/GTATGT -> AAG/GTAAGT

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RP51A-NoBS-NoPseudoBS

    Plasmid

    #100986

    Purpose

    RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. U6-U57A

    Plasmid

    #169927

    Purpose

    WT U6 with a U57A mutation

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    U6 U57A
    

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pG-02

    Plasmid

    #166933

    Purpose

    Target AD1.A5 part (G)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Star sense AD1.S5 +RBS - Block G- Flanked by BsaI restriction sites
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC-03

    Plasmid

    #166927

    Purpose

    STAR-AD1.A6 part (C)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Star Antisense AD1.A6 - Block C - Flanked by BsaI sites
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC-02

    Plasmid

    #166926

    Purpose

    STAR-AD1.A5 part (C)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Star Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVG50

    Plasmid

    #164708

    Purpose

    yEVenus under the control of tetOpr with ADH1t upstream of the reporter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t
    

    Expression

    Yeast

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hsh155 Hs 1-16 3x FLAG

    Plasmid

    #111966

    Purpose

    Human HEATs 1-16 have been inserted into Hsh155 3xFLAG.

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 Hs 1-16 3x FLAG
    

    Tags

    3xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Human HEATs 1-16 inserted

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Hsh155 Hs 1-12 3xFLAG

    Plasmid

    #111965

    Purpose

    Human HEATs 1-12 have been inserted into Hsh155 3xFLAG.

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

    Insert

    Hsh155 Hs 1-12 3x FLAG
    

    Tags

    3xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Human HEATs 1-12 inserted

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge