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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,837 results for: pET

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Showing: 6881 - 6900 of 9837 results

  3. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  4. pTCF176 HLA-A*02:01 (CD8 mut)

    Plasmid
    #180459
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01 (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, T228A, replacement of transmembrane domain…
    Available Since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180459-plasmid-map-sequence-id-355125

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  5. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  6. pKK-TEV-mEGFP

    Plasmid
    #105794
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mEGFP
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105794-plasmid-map-sequence-id-211381

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  7. pKK-TEV-mTagBFP

    Plasmid
    #105789
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mTagBFP
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105789-plasmid-map-sequence-id-203380

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  8. 4YZ8

    Plasmid
    #69955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. BRPF3

    Plasmid
    #28269
    Depositor
    Cheryl Arrowsmith
    Insert
    BRPF3 (BRPF3 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP domain (residues 1056-1195).
    Available Since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. PPIE

    Plasmid
    #25605
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPIE (PPIE Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 131-301.
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. NMT1 (3IU2)

    Plasmid
    #25272
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NMT1:R115-Q496 (NMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids R115-Q496
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. NMT1 (3JTK)

    Plasmid
    #25277
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NMT1:T108-Q496 (NMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids T108-Q496
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. UBA05 (3GUC)

    Plasmid
    #25212
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    uba05.057.329 (UBA5 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  15. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

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  16. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available Since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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  18. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  20. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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Showing: 6881 - 6900 of 9837 results