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We narrowed to 9,634 results for: 11

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Showing: 6961 - 6980 of 9634 results

  1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  2. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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  3. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-488812

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  5. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  8. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid
    #224356
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)
    Depositor
    Nicolas Charlet-Berguerand
    Article
    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.
    Insert
    uN2C with a GGC repeat expansion (100x)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intron (CAG)
    Available Since
    Feb. 5, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224356-plasmid-map-sequence-id-460492

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  12. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  15. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114084-plasmid-map-sequence-id-230536

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  18. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114082-plasmid-map-sequence-id-220504

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  19. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid
    #114081
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114081-plasmid-map-sequence-id-231038

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Showing: 6961 - 6980 of 9634 results