We narrowed to 7,274 results for: 11

1. His-GST-Cys-M87 Spastin

   Plasmid

   #184804

   Purpose

   Bacterial expression of M87 Spastin (A2C mutation)

   Depositor

   James Hurley

   Article

   Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

   Insert

   M87 Spastin (SPAST Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   A2C

   Promoter

   T7

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426_Cas9_gRNA-ARS1114a

   Plasmid

   #87397

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1114a sequence CTTGTGAAACAAATAATTGG in yeast chromosome 11.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1114a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pMSCV-PIG-Lin28b

   Plasmid

   #64795

   Purpose

   Expresses human Lin28b in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Lin28b (LIN28B Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGLS3-5xHRE-PHLDA3(wt)

   Plasmid

   #72557

   Purpose

   Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   PHLDA3 (PHLDA3 Human)
   

   Use

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   Promoter

   E1b minimal

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest27-mBIN1+17

   Plasmid

   #61096

   Purpose

   Express N-terminal GST tagged-mouse BIN1+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-nAChr-Alpha6

   Plasmid

   #48812

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRevTRE MKL1-N100 S449A/T450A/S454A

   Plasmid

   #19849

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1-N100 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   deleted amino acids 1-100 and changed Serine 449,…

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

   Plasmid

   #26577

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDN-G1TGt

    Plasmid

    #44508

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::yEGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pGFP-Kif5cmut

    Plasmid

    #71854

    Purpose

    mammalian expression of mutant GFP-Kif5c

    Depositor

    Michelle Peckham

    Article

    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T93N mutation and also a novel SpeI site (1652). …

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pGL3-IRES-Lin28b-P3

    Plasmid

    #64794

    Purpose

    Luciferase expression driven by Lin28b promoter P3

    Depositor

    Joshua Mendell

    Article

    Chang et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Lin28b promoter P3 (LIN28B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1_MeCP2(R111G)

    Plasmid

    #110187

    Purpose

    Expression of mouse MeCP2 (R111G) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arg 111 Gly

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPPAT-11AA-2XStrep

    Plasmid

    #125680

    Purpose

    expresses human PPAT protein, with first 11 residues removed, with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    the first eleven residues have been removed

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gal4-VP16-HSF1 WT

    Plasmid

    #71726

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1 WT RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 VP16 AD (HSF1 Budding Yeast, Human, Herpes Simplex Virus protein VP16)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. integrin beta6 777T pcDNA1 neo

    Plasmid

    #13582

    Depositor

    Dean Sheppard

    Article

    Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

    Insert

    integrin beta 6 777T (ITGB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    777T. Lacks last 11 amino acids of cytoplasmic do…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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