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pWL89A mmPing20F

Plasmid

#145789

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Yeast

Mutation

Insertion of the FMV enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20B

Plasmid

#145790

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20B

Expression

Yeast

Mutation

Insertion of the b-Conglycin Enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A NL60

Plasmid

#145791

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

NL60

Expression

Yeast

Mutation

Insertion of the OCS Enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A 2xE

Plasmid

#145792

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

2xE

Expression

Yeast

Mutation

Insertion of 2 copies of the CMV Enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A 4xE

Plasmid

#145793

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

4XE

Expression

Yeast

Mutation

Insertion of 4 copies of the CMV Enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Publication

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Publication

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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GGE207

Plasmid

#120792

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown-Mfe

Use

Cloning/assembly plasmid

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ292

Plasmid

#102686

Purpose

S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Spt2-K166R-GFP

Plasmid

#115573

Purpose

Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166

Depositor

Michael Downey

Publication

Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

Insert

SPT2

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Lysine 166 mutated to Arginine

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only
pRS315-PGK-SEIPIN2

Plasmid

#96987

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

Depositor

Kent Chapman

Publication

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Expression

Yeast

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PP76

Plasmid

#78946

Purpose

Supplemental Figure 3. Integration Site 3.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 3

Expression

Yeast

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP318

Plasmid

#78941

Purpose

Expresses GFP. Figure 3. Strain 5.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP322

Plasmid

#78943

Purpose

Expresses GFP. Figure 3. Strain 9.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP67

Plasmid

#78951

Purpose

Supplemental Figure 3. Integration Site 8.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 8

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP151

Plasmid

#78952

Purpose

Supplemental Figure 3. Integration Site 9.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 9

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP297

Plasmid

#78937

Purpose

Expresses GFP. Figure 3. Strain 3.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP298

Plasmid

#78938

Purpose

Expresses GFP. Figure 3. Strain 4.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP299

Plasmid

#78939

Purpose

Expresses GFP. Figure 3. Strain 6.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP300

Plasmid

#78940

Purpose

Expresses GFP. Figure 3. Strain 7.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge