Purpose
All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
Inserts
O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
mCherry
ωRNA
Tags
HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
Mutation
R-IscB: changed Aspartic Acid 60 to Alanine, chan…
Available Since
Nov. 13, 2025
Availability
Academic Institutions and Nonprofits only