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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 6981 - 7000 of 9957 results

  1. NMT1 (3IU2)

    Plasmid
    #25272
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NMT1:R115-Q496 (NMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids R115-Q496
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. NMT1 (3JTK)

    Plasmid
    #25277
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NMT1:T108-Q496 (NMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids T108-Q496
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. UBA05 (3GUC)

    Plasmid
    #25212
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    uba05.057.329 (UBA5 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  5. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Publication
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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  7. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  9. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72400-plasmid-map-sequence-id-133994

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  10. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  11. TRIM54

    Plasmid
    #28277
    Depositor
    Cheryl Arrowsmith
    Insert
    TRIM54 (TRIM54 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    B-Box domain, residues 122-168.
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. NKTR

    Plasmid
    #25597
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NKTR (NKTR Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  13. UPLC1

    Plasmid
    #25550
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UPLC1 (ASAP3 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. SULT4A1

    Plasmid
    #25480
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SULT4A1 (SULT4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. LT2_14_GlyOx

    Plasmid
    #177711
    Purpose
    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)
    Depositor
    Lisbeth Olsson
    Publication
    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d
    Inserts
    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    Use
    Used as donor dna in genome integration after lin…
    Expression
    Yeast
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177711-plasmid-map-sequence-id-378782

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Showing: 6981 - 7000 of 9957 results