We narrowed to 11,022 results for: ENA;

1. mCerulean-PCNA-HygroR

   Plasmid

   #215064

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

   Plasmid

   #100716

   Purpose

   B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting ATP1A1 (cloned using BsmBI)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1.shCDK8

   Plasmid

   #19760

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   small hairpin RNA against CDK8 (CDK8 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-CB1-HIF2a-GFP-T2A-Puro

   Plasmid

   #71708

   Purpose

   Lentiviral expression of HIF2A-GFP

   Depositor

   Eric Jonasch, Xiande Liu

   Article

   Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

   Insert

   HIF2A (EPAS1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SspB-HaloTag-DHPH-ITSN1

   Plasmid

   #176113

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75240

   Purpose

   CRISPR/Cas9 plasmid against human Ikaros

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

   Plasmid

   #188758

   Purpose

   Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   VP64
   

   Use

   Lentiviral

   Tags

   rTetR(SE-G72P)-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75241

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR ErbB2-CD3ε-FusionRed

    Plasmid

    #188628

    Purpose

    Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    ERBB2 (ERBB2 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    CD3ε-FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-HIF2α P405A/P531A/N847A-pBabe-Puro

    Plasmid

    #25956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

    Plasmid

    #69048

    Purpose

    lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 1, Q146H

    Promoter

    CAG

    Available Since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-PA-Rac1-I539E

    Plasmid

    #22026

    Purpose

    Expression of photoactivatable Rac1 ('lit' state mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains 'lit state' mutatio…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. Anti-NALCN [N185/7R]

    Plasmid

    #177508

    Purpose

    Mammalian Expression Plasmid of anti-NALCN (Rat). Derived from hybridoma N185/7.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-NALCN (Rattus norvegicus) recombinant mouse monoclonal antibody (Nalcn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SspB-iRFP-DHPH-ITSN1

    Plasmid

    #176117

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-Parvalbumin [L114/3R]

    Plasmid

    #177459

    Purpose

    Mammalian Expression Plasmid of anti-Parvalbumin (Rat). Derived from hybridoma L114/3.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody (Pvalb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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