Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 26,289 results for: nes

Clear keywords
Showing: 7001 - 7020 of 26289 results

  1. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

    Enlarge

  2. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid.
    Depositor
    Johannes Stuttmann
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

    Enlarge

  7. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

    Enlarge

  8. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

    Enlarge

  9. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

    Enlarge

  10. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

    Enlarge

  11. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

    Enlarge

  12. pAB1923 REPAIR.t1-S

    Plasmid
    #176321
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q/E620G/Q696L, only the deaminase domain (aa …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176321-plasmid-map-sequence-id-345288

    Enlarge

  13. AAV_pMBP-EGFP-caax (AAV8)

    Viral Prep
    #190155-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from AAV_pMBP-EGFP-caax (#190155). In addition to the viral particles, you will also receive purified AAV_pMBP-EGFP-caax plasmid DNA. MBP-driven EGFP expression in oligodendrocytes. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brad Zuchero
    Article
    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:
    Promoter
    MBP
    Available Since
    Feb. 6, 2026
    Availability
    Academic Institutions and Nonprofits only

  14. AAV_pMBP-EGFP-caax (AAV1)

    Viral Prep
    #190155-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV_pMBP-EGFP-caax (#190155). In addition to the viral particles, you will also receive purified AAV_pMBP-EGFP-caax plasmid DNA. MBP-driven EGFP expression in oligodendrocytes. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brad Zuchero
    Article
    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:
    Promoter
    MBP
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only

  15. RF21

    Bacterial Strain
    #102803
    Purpose
    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)
    Depositor
    Robert Gennis, Toshio Iwasaki
    Article
    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d
    Bacterial Resistance
    None
    Available Since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-EFS-SpCas9 (AAV8)

    Viral Prep
    #104588-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EFS-SpCas9 (#104588). In addition to the viral particles, you will also receive purified pAAV-EFS-SpCas9 plasmid DNA. EFS-driven expression of Myc-tagged SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ryohei Yasuda
    Article
    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)
    Promoter
    EFS
    Available Since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
Showing: 7001 - 7020 of 26289 results