pLenti CMV TRE3G Neo GFP-Progerin C661S
(Plasmid #118711)

• Purpose: Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)
• Depositing Lab: Tom Misteli
• Publication: Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 10.1016/j.ymeth.2015.08.024.

Backbone

• Vector backbone: pLenti CMV TRE3G Neomycin
• Backbone manufacturer: Eric Campeau
• Backbone size w/o insert (bp): 8013
• Total vector size (bp): 10645
• Vector type: Mammalian Expression, Lentiviral ; TET-ON inducible
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Construct may be grown in DH5A cells, and or 37 degrees, though STBL3 and 30 degrees are recommended to reduce chance of recombination events.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP-Progerin C661S
• Alt name: Progerin C661S
• Species: H. sapiens (human)
• Insert Size (bp): 2632
• Mutation: C661S farnesylation mutant
• GenBank ID: ENSG00000160789
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV TRE3G (TET-ON)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: tgaccagtttactccctatcagtg
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate mammalian inducible cell lines co-infect with lentivirus made from:
pLenti CMV rtTA3 Hygro (w785-1)( Addgene Plasmid #26730)