We narrowed to 62,316 results for: cloning

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pVenusCfN

Plasmid

#128566

Purpose

Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.

Depositor

Oliver Vugrek

Article

Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11

Insert

Venus

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT.000

Plasmid

#119559

Purpose

Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-GST-3C-LIC

Plasmid

#108710

Purpose

Expression of a GST-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMB.PRC2

Plasmid

#234579

Purpose

Expression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Insect

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-NatMx

Plasmid

#122918

Purpose

ADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-HphMx

Plasmid

#122916

Purpose

ADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFH56

Plasmid

#125882

Purpose

Level0 Rice OsACT1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsAct1 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH22

Plasmid

#125808

Purpose

Level0 xCas9 3.7

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

xCas9 3.7

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Rat SERCA3 (pMT2)

Plasmid

#75185

Purpose

Mammalian expression of ATP2A3

Depositor

Jonathan Lytton, Gary Shull

Article

Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

Insert

ATP2A3 (Atp2a3 Rat)

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGGF001

Plasmid

#48842

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::BastaR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti sgRNA CFP

Plasmid

#155281

Purpose

Lentiviral sgRNA cloning with CFP marker

Depositor

Martin Carroll

Type

Empty backbone

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)

Plasmid

#242591

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

Plasmid

#229845

Purpose

A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin nanobody

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#67964

Purpose

Mobilizible plasmid with EGFP. OriT from the F plasmid.

Depositor

Joshua Leonard

Insert

OriT

Use

Synthetic Biology

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pME Integrin beta 1b

Plasmid

#105976

Purpose

entry clone for gateway cloning, Full length Integrin beta 1b without stop

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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UBC64

Plasmid

#25143

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubc64.248.414 (UBE2Q1 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Mutation

contains UBC domain; residues 248-414

Available Since

Oct. 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits
CDYL

Plasmid

#25509

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CDYL (CDYL Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits