We narrowed to 7,766 results for: loxP

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RAP2A-p2A-E2Crimson

Plasmid

#83940

Purpose

Lentiviral vector expressing RAP2A-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SLC35B2-p2A-E2Crimson

Plasmid

#83941

Purpose

Lentiviral vector expressing SLC35B2-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

Plasmid

#159907

Purpose

Mutagenesis of Netrin1

Depositor

Larry Zweifel

Insert

Netrin1 (Ntn1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045E

Plasmid

#125007

Purpose

Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Publication

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Lentiviral human Citron shRNA 1 RFP i670

Plasmid

#155345

Purpose

Lentiviral expression of humnan CIT shRNA, RFP i670 expression, based on Addgene 12247

Depositor

Martin Carroll

Insert

Citron (CIT Human)

Expression

Mammalian

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Dox human Citron shRNA 1 GFP

Plasmid

#155291

Purpose

Lentivirus for doxycycline inducible expression of human Citron shRNA, GFP marker, based on Addgene 11652

Depositor

Martin Carroll

Insert

Citron (CIT Human)

Expression

Mammalian

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-3'short

Plasmid

#129749

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'short

Plasmid

#129748

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP

Plasmid

#129747

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e1-EGFP

Plasmid

#129751

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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frameshift ELL2

Plasmid

#127257

Purpose

For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

Depositor

Christine Milcarek

Publication

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

XhoI site between the 1st &2nd Met was filled…

Promoter

T7

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Tra].1046D

Plasmid

#112693

Purpose

express two gRNA targeting bTub & Tra under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP2

Plasmid

#114300

Purpose

Constitutive expression of the TFDP2 protein

Depositor

Patrick Salmon

Publication

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP2 (TFDP2 Human)

Use

Lentiviral

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-EpoRtm

Plasmid

#114286

Purpose

TET-inducible expression of Erythropoietin receptor without cytoplasmic tail

Depositor

Patrick Salmon

Insert

Erythropoietin Receptor (Epor Rat)

Use

Lentiviral

Mutation

deletion of the cytoplasmic domain

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Publication

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Publication

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Promoter

CAG

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 S1338A

Plasmid

#111808

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Publication

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (TSC2 Fly)

Tags

3xFlag

Expression

Insect

Mutation

S1338A

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-shScn4b-268

Plasmid

#99031

Purpose

Expresses a shRNA targeting the mouse Scn4b gene

Depositor

Amy Lasek

Publication

Ji et al Front Cell Neurosci. 2017 Feb 13;11:17.

Insert

Scn4b shRNA (Scn4b Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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