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pAF9D94 (pAF10C6)

Plasmid

#185848

Purpose

Expressing artificial trans-repressor in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>CYC8>PABF1

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK035

Plasmid

#180827

Purpose

T7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

SwGdmB

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-G1pCNPRS

Plasmid

#174719

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XII2up

Plasmid

#176174

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XII2dw

Plasmid

#176175

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-2

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC6-spacer

Plasmid

#176178

Purpose

yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

41 bp non-coding DNA spacer from pYTK048

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJAID8Z105

Plasmid

#165057

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL:P3S + STS:P4S

Plasmid

#139789

Purpose

expression of biosynthetic enzymes 4CL and STS in fusion with CC

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

biosynthetic enzymes 4CL and STS in fusion with coiled-coil segments

Expression

Bacterial

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD2 probe

Plasmid

#73070

Purpose

antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD2 (SNORD2 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD60 probe

Plasmid

#73071

Purpose

antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD60 (SNORD60 Human)

Expression

Mammalian

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pRSETA-Prp28

Plasmid

#111285

Purpose

Prp28 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP28

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-14

Plasmid

#111311

Purpose

snu114-14

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Mutation

snu114-14

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pIR195

Plasmid

#64810

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pETcc/NuG2-RTX-i-v-RS1137-NuG2

Plasmid

#259689

Purpose

E. coli expression plasmid encoding CyaA RTX repeats I–V with an RS insertion between RTX-i and RTX-ii for optical-tweezers studies of sequential RTX folding.

Depositor

Hongbin Li

Article

Chen et al J Biol Chem. 2023 Sep;299(9):105150. doi

Insert

NuG2-RTX-i-v-RS1137-NuG2

Expression

Bacterial

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pETcc/NuG2-RTX-i-v-RS1261-NuG2

Plasmid

#259688

Purpose

E. coli expression plasmid encoding CyaA RTX repeats I–V with an RS1261 insertion between RTX-II and RTX-III, flanked by NuG2 domains for optical-tweezers studies.

Depositor

Hongbin Li

Article

Chen et al J Biol Chem. 2023 Sep;299(9):105150. doi

Insert

NuG2-RTX-i-v-RS1261-NuG2

Expression

Bacterial

Available Since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-EGFP

Plasmid

#252340

Purpose

Transient expression of EGFP.

Depositor

James Conway

Article

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-His-SUMO-TlaA2E20D

Plasmid

#257744

Purpose

For expression and co-expression with the modifying enzyme in the pathway, TlaM

Depositor

Wilfred van der Donk

Article

Weir et al bioRxiv [Preprint]. 2026 Apr 4:2026.04.0

Insert

TlaA2

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

E20D

Promoter

T7

Available Since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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