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pDMJ2_SARS2_S_Brigham_Day143

Plasmid

#256962

Purpose

Expresses SARS-CoV-2 spike (Brigham Day 143) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

P9L, Δ142GVY144, Q183H, T478K, Y489H, S494P, N501…

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2_SARS2_S_Brigham_Day75

Plasmid

#256958

Purpose

Expresses SARS-CoV-2 spike (Brigham Day 75) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

Δ142GVY144, T478K, E484K, S494P, D614G, I870V

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2_SARS2_S_Brigham_Day75_D614

Plasmid

#256959

Purpose

Expresses SARS-CoV-2 spike (Brigham Day 75 D614) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

Δ142GVY144, T478K, E484K, S494P, I870V

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2_SARS2_S_WuhanHu1

Plasmid

#256956

Purpose

Expresses SARS-CoV-2 spike (WuhanHu1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

None

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2_SARS2_S_WuhanHu1_D614G

Plasmid

#256957

Purpose

Expresses SARS-CoV-2 spike (WuhanHu1 D614G) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-S2 (JDW 1529)

Plasmid

#242605

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-S2. H2B for nuclear labeling

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-S2

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-H (JDW 1530)

Plasmid

#242604

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-H. H2B for nuclear labeling

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-H

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Sun1-2xsfGFP-6xMYC-pA (JDW 694)

Plasmid

#242588

Purpose

A CMV driven expression vector containing a Sun1-2xsfGFP for labeling nuclear envenlope.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC (Sun1 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

Plasmid

#229827

Purpose

A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xnls-TagBFP2-MCS-FLAG

Expression

Mammalian

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pALS099 (pGEX-2TP-cTevHis-DEST)

Plasmid

#139787

Purpose

Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Jeannie Lee, Arneet Saltzman

Publication

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available since

April 23, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Publication

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Publication

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Publication

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway Cloning and Luciferase

Promoter

SV40

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR221_SLC19A1

Plasmid

#132266

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221_SLC27A4

Plasmid

#132283

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A4 (SLC27A4 Human)

Expression

Mammalian

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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