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pTaCas9-H840A_5

Plasmid

#91172

Purpose

Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A (nickase)

Use

CRISPR

Expression

Plant

Mutation

H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_6

Plasmid

#91171

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_3

Plasmid

#91161

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRF2ZNF1-11

Plasmid

#87178

Purpose

Retroviral stable expression of chimeric protein TRF2ZNF1-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

chimeric protein TRF2ZNF1-11

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt2(K14A)

Plasmid

#86624

Purpose

Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A) (AKT2 )

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)

Plasmid

#70740

Purpose

plasmid for building 3n2, 5n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)

Plasmid

#70736

Purpose

plasmid for building 3n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC102

Plasmid

#62337

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY)

Plasmid

#251547

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-P2A-GFP

Plasmid

#247161

Purpose

In vitro transcription of human codon optimized wildtype Dn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4denV

Plasmid

#228216

Purpose

Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme

Depositor

Stephen Benkovic

Insert

denV

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_CMV_HA-VSVG

Plasmid

#212645

Purpose

Lentivirus vector to express HA-VSVG

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

VSVG

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_ZeoR

Plasmid

#212649

Purpose

piggyBac vector to express FLAG-tNGFR with zeocin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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SL2 GFP H2B

Plasmid

#227717

Purpose

Plasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

SL2 GFP H2B (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAM282

Plasmid

#226121

Purpose

Expression of cdc48

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 (CDC48 Budding Yeast)

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLPR2

Plasmid

#209980

Purpose

Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (cat)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPO5

Plasmid

#209987

Purpose

Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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