BioBits Explorer: A modular synthetic biology education kit.
Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug. PubMed Article
Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106285 | pJL1 Aquamarine | Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter |
| 106286 | pJL1 ATF1 | Expresses ATF1 in Ecoli off a T7 promoter |
| 106287 | pJL1 BSMT1 | Expresses BSMT1 in Ecoli off a T7 promoter |
| 106288 | pJL1 sortase | Expresses sortase in Ecoli off a T7 promoter |
| 106289 | pNL1 Ecarin | Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector |
| 106290 | pJL1 Trx-Bx | Expresses Trx-Bx in Ecoli off a T7 promoter |
| 106320 | pJL1 eforRed | Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter |
| 107353 | pNP1 eforRed toehold switch | Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed |
| 107354 | pNP1 tdTomato toehold switch | Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed |
| 107355 | pNP1 sfGFP toehold switch | Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed |
| 107356 | pNP1 Aqua toehold switch | Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed |
| 107357 | pNP1 eforRed trigger | Trigger sequence for pNP1 eforRed toehold switch |
| 107358 | pNP1 tdTomato trigger | Trigger sequence for pNP1 tdTomato toehold switch |
| 107359 | pNP1 sfGFP trigger | Trigger sequence for pNP1 sfGFP toehold switch |
| 107360 | pNP1 Aqua trigger | Trigger sequence for pNP1 Aqua toehold switch |
| 107361 | pNP1 AND gate sfGFP | Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed |
| 107362 | pNP1 AND input A | Input A for pNP1 AND gate sfGFP |
| 107363 | pNP1 AND input B | Input B for pNP1 AND gate sfGFP |
| 107364 | pNP1 OR sfGFP | Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed |
| 107365 | pNP1 OR input A | Input A for pNP1 OR gate sfGFP |
| 107366 | pNP1 OR input B | Input B for pNP1 OR gate sfGFP |
| 107367 | pCOLA banana sensor sfGFP | Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA |
| 107368 | pCOLA kiwi sensor sfGFP | Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA |