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BioBits Explorer: A modular synthetic biology education kit.
Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ
Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
106285pJL1 AquamarineExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter
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106286pJL1 ATF1Expresses ATF1 in Ecoli off a T7 promoter
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106287pJL1 BSMT1Expresses BSMT1 in Ecoli off a T7 promoter
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106288pJL1 sortaseExpresses sortase in Ecoli off a T7 promoter
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106289pNL1 EcarinExpresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector
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106290pJL1 Trx-BxExpresses Trx-Bx in Ecoli off a T7 promoter
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106320pJL1 eforRedExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter
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107353pNP1 eforRed toehold switchExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed
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107354pNP1 tdTomato toehold switchExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed
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107355pNP1 sfGFP toehold switchExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed
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107356pNP1 Aqua toehold switchExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed
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107357pNP1 eforRed triggerTrigger sequence for pNP1 eforRed toehold switch
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107358pNP1 tdTomato triggerTrigger sequence for pNP1 tdTomato toehold switch
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107359pNP1 sfGFP triggerTrigger sequence for pNP1 sfGFP toehold switch
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107360pNP1 Aqua triggerTrigger sequence for pNP1 Aqua toehold switch
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107361pNP1 AND gate sfGFPExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 AND input A and pNP1 AND input B are co-expressed
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107362pNP1 AND input AInput A for pNP1 AND gate sfGFP
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107363pNP1 AND input BInput B for pNP1 AND gate sfGFP
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107364pNP1 OR sfGFPExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed
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107365pNP1 OR input AInput A for pNP1 OR gate sfGFP
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107366pNP1 OR input BInput B for pNP1 OR gate sfGFP
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107367pCOLA banana sensor sfGFPExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA
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107368pCOLA kiwi sensor sfGFPExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA
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