pJL1 Trx-Bx
(Plasmid #106290)

• Purpose: Expresses Trx-Bx in Ecoli off a T7 promoter
• Depositing Lab: James Collins
• Publication: Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug.

Backbone

• Vector backbone: pJL1
• Backbone manufacturer: Michael Jewett Lab
• Backbone size w/o insert (bp): 2724
• Total vector size (bp): 3261
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trx-BX
• Alt name: Thioredoxin; Bx (Serine protease domain)
• Insert Size (bp): 1071
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAAATTAATACGACTCACTATAGGGAGACC
• 3′ sequencing primer: GCTCGAGGTTATCCGGATATAGTTC

Resource Information

• Supplemental Documents:
  • pJL1 Trx-Bx.gb
• A portion of this plasmid was derived from a plasmid made by: Trx: Uniprot: P0AA25, Bx: UniProt: P04971.1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry