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pJL1 Aquamarine
(Plasmid #106285)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 106285 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJL1
  • Backbone manufacturer
    Michael Jewett Lab
  • Backbone size w/o insert (bp) 2724
  • Total vector size (bp) 2982
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aquamarine
  • Alt name
    ECFP-T65S-H148G
  • Species
    A. victoria
  • Insert Size (bp)
    792
  • Mutation
    T65S and H148G
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis and TEV cleavage (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAAATTAATACGACTCACTATAGGGAGACC
  • 3′ sequencing primer GCTCGAGGTTATCCGGATATAGTTC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL1 Aquamarine was a gift from James Collins (Addgene plasmid # 106285 ; http://n2t.net/addgene:106285 ; RRID:Addgene_106285)
  • For your References section:

    BioBits Explorer: A modular synthetic biology education kit. Huang A, Nguyen PQ, Stark JC, Takahashi MK, Donghia N, Ferrante T, Dy AJ, Hsu KJ, Dubner RS, Pardee K, Jewett MC, Collins JJ. Sci Adv. 2018 Aug 1;4(8):eaat5105. doi: 10.1126/sciadv.aat5105. eCollection 2018 Aug. 10.1126/sciadv.aat5105 PubMed 30083608