We narrowed to 13,804 results for: gan

1. rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

   Plasmid

   #32481

   Purpose

   Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F,Y115F-GlyR
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Promoter

   Synapsin

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-blueAKAR(T/A)

   Plasmid

   #118471

   Purpose

   Negative-control mutant for blueAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueAKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

   Plasmid

   #182353

   Purpose

   GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nanog-2A-GFP

   Plasmid

   #59994

   Purpose

   Inserts GFP immediately downstream of the Nanog coding region

   Depositor

   Rudolf Jaenisch

   Article

   Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2A-GFP

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-GST-4

   Plasmid

   #228986

   Purpose

   For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-4
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL0341 (pTarget)

   Plasmid

   #200906

   Purpose

   Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   pTarget
   

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU1-B-SP44s-GUS

   Plasmid

   #172430

   Purpose

   Expresses Streptomyces codon-optimised beta-D-glucuronidase

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   beta-D-glucuronidase
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   SP44s

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mDlx-SynTagMA_post-2A-mCerulean

    Plasmid

    #130899

    Purpose

    Synaptic Tag for Mapping Activity in GABAergic neurons

    Depositor

    Simon Wiegert

    Article

    Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

    Insert

    SynTagMA
    

    Use

    AAV

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5 mSynCAM 2

    Plasmid

    #115497

    Purpose

    expression of mouse full-length SynCAM 2

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm2 (Cadm2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHES830

    Plasmid

    #87944

    Purpose

    Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
    

    Expression

    Yeast

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 3xFlag IFIT5

    Plasmid

    #53556

    Purpose

    mammalian expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SG3ΔENV CA E45A

    Plasmid

    #145783

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residue glutamic acid 45 to alanin…

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIRECT_21A

    Plasmid

    #91128

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human V5-USP47

    Plasmid

    #217626

    Purpose

    Mammalian expression of V5 tagged USP47

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    USP47 (USP47 Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ZJOM40

    Plasmid

    #133650

    Purpose

    Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-SEC7-DuDre (SEC7 Budding Yeast)
    

    Tags

    DuDre

    Expression

    Yeast

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZJOM140

    Plasmid

    #133668

    Purpose

    Integration of COX4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-mTagBFP2 (COX4 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-EF1-IRES-NEO deleted ITR

    Plasmid

    #67906

    Purpose

    PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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