We narrowed to 7,649 results for: Pol

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p35S-FHA-AtAGO4_gDNA

Plasmid

#216838

Purpose

N-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoter

Depositor

Arturo Marí-Ordóñez

Article

Dombey et al Genome Res. 2025 Mar 18;35(3):522-544. d

Insert

ARGONAUTE4 (AGO4 Mustard Weed)

Tags

Flag-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pTag4C_AVPR1A-V2R-NTEV-tcs-GV

Plasmid

#227102

Purpose

AVPR1A receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

AVPR1A-NTEV (AVPR1A Human)

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_ADRA1A-V2R-NTEV-tcs-GV

Plasmid

#227101

Purpose

ADRA1A receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

ADRA1A-NTEV (ADRA1A Human)

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

Plasmid

#169135

Purpose

E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Coh7

Tags

3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_WDR13:142-485

Plasmid

#210924

Purpose

Insect expression of human WDR13 fragment A142 to K485. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR13:A142-K485 (WDR13 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

H325R natural variant (VAR_060284)

Promoter

Polyhedrin

Available Since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_EIF3I:1-325

Plasmid

#210910

Purpose

Insect expression of full-length human EIF3I. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3I:M1-A325 (EIF3I Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_COP1:386-731

Plasmid

#210887

Purpose

Insect expression of human COP1 fragment A386 to V731. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COP1:A386-V731 (COP1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_PRPF4:1-522

Plasmid

#210896

Purpose

Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRPF4:M1-E522 (PRPF4 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_CORO1A:1-461

Plasmid

#210891

Purpose

Insect expression of full-length human CORO1A. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR91:392-747

Plasmid

#210880

Purpose

Insect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWL

Plasmid

#204814

Purpose

Bacterial expression of MERS Mpro

Depositor

ASAP Discovery, Frank von Delft

Insert

MERS Mpro (orf1ab MERS)

Expression

Bacterial

Mutation

MSAVLQ/SGLVKM

Available Since

Feb. 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

Plasmid

#204803

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PABPC1_544-626_pGEX6P1

Plasmid

#209790

Purpose

Bacterial expression of PABPC1 Mlle domain

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

Insert

Mlle domain of PABPC1 (PABPC1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 544-626 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

Plasmid

#201466

Purpose

Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 111-136

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

Plasmid

#201467

Purpose

Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 137-160

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

Plasmid

#201468

Purpose

Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 202-230

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

Plasmid

#201469

Purpose

Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 287-316

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

Plasmid

#196878

Purpose

Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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