We narrowed to 157,817 results for: ins

1. MSP2279

   Plasmid

   #70706

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A and H557A in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Syk(NC)-SH2

   Plasmid

   #46521

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Syk(NC) (SYK Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 7-263

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGen2.1-redCALWY-1

   Plasmid

   #50739

   Purpose

   Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

   Insert

   gene for redCALWY-1 Zn2+ sensor
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-ReaChR-citrine

   Plasmid

   #169045

   Purpose

   Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-citrine
   

   Use

   Gateway entry cloning vector

   Tags

   citrine

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFKBP-myc

   Plasmid

   #20213

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFKBP (FKBP1A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pGEX PLCg1(C)-SH2

   Plasmid

   #46469

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   PLCg1(C)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 656-766

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-GP-2-MPHOSPH8 Chromo

   Plasmid

   #59694

   Purpose

   Contains histone modification interacting domain (MPHOSPH8 Chromo)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   MPHOSPH8 Chromo (MPHOSPH8 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Sept. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNP1 Aqua toehold switch

   Plasmid

   #107356

   Purpose

   Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 8 + Aquamarine
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. E. coli 16S toehold switch sensor

   Plasmid

   #110698

   Purpose

   Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   E. coli 16S toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLTR PKC beta II

    Plasmid

    #8417

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Rat Protein Kinase C beta II (Prkcb Rat)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

12. B. fragilis 16S toehold switch sensor

    Plasmid

    #110696

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Vav2-SH2

    Plasmid

    #46529

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 665-774

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a Gx-NL3

    Plasmid

    #169108

    Purpose

    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pExpreS2-1-C3-PA

    Plasmid

    #175446

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-Protein A and BiP Secretion Peptide

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Syk(N)-SH2

    Plasmid

    #46520

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(N) (SYK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 7-114

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+) 6xHis-TEV-IFIT5

    Plasmid

    #53560

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLTR PKC delta

    Plasmid

    #8419

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C delta (Prkcd Mouse)
    

    Expression

    Mammalian

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-R2ACR_142-EYFP

    Plasmid

    #103964

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R2ACR_142
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xFKBP-myc

    Plasmid

    #20212

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFKBP (FKBP1A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only