We narrowed to 23,322 results for: tes

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pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-L1374

Plasmid

#226278

Purpose

Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMS19

Plasmid

#229475

Purpose

Expresses RssB with E135A, E136A, & E137A mutation

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS21

Plasmid

#229476

Purpose

Expresses RssB with E135A, E136A, & E137A mutation with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E135A, E136A, E137A)

Tags

10xHis

Expression

Bacterial

Mutation

E135A, E136A, E137A

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF18

Plasmid

#229456

Purpose

Expresses IraD (aa17-130) with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD (aa 17-130 only)

Tags

6xHis

Expression

Bacterial

Mutation

aa 17-130 only

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-NCYC110

Plasmid

#226273

Purpose

Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-L1374

Plasmid

#226271

Purpose

Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAM227

Plasmid

#226650

Purpose

MSS-His6-UFD1L

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

UFD1 (UFD1 Human)

Tags

His-thrombin

Expression

Bacterial

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_LmFPPS

Plasmid

#186322

Purpose

pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

Insert

FPPS

Tags

His Tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-tractin

Plasmid

#187649

Purpose

Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

F-tractin

Use

AAV

Tags

mNeonGreen

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc0---MS2

Plasmid

#210572

Purpose

Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-SHPmut-FLAG

Plasmid

#225502

Purpose

Inducible expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-WT-FLAG

Plasmid

#225501

Purpose

Inducible expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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