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pLPC-Flag-BirA-Linker-Myc-mRap1

Plasmid

#184644

Purpose

Retroviral expression of BirA-mRap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

BirA-rap1

Use

Retroviral

Tags

BirA FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p23-NES-RanCas13b-msfGFP-NES-Flag

Plasmid

#165073

Purpose

overexpression of RanCas13b in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RanCas13b

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

Plasmid

#169960

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

Plasmid

#169959

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

Plasmid

#169961

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

Plasmid

#170119

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-N-Myc-Flag-BirA-hPOT1-DeltaOB

Plasmid

#166410

Purpose

express BirA-hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Tags

MYC-flag-BirA

Expression

Mammalian

Mutation

ΔOB

Promoter

cmv

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-019 FLAG IFP1.4[1] IRES IFP1.4[2]

Plasmid

#130129

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

IFP1.4

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-020 FLAG miRFP670[1] IRES miRFP670[2]

Plasmid

#130130

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

miRFP670

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

Plasmid

#127409

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

Plasmid

#127406

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

Plasmid

#127402

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

Plasmid

#127400

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (delta200)

Plasmid

#120278

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the delta200 Golgi-…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLex-FLAG-FASTKD4 mutant delta 226

Plasmid

#73621

Purpose

Expression vector containing FASTKD4 mutant delta 226

Depositor

Vamsi Mootha

Article

Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

Insert

FASTKD4 (TBRG4 Human)

Mutation

Y616A, L617A, K618A, K620A

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 L128C/H113E

Plasmid

#111778

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L128C/H113E

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 T336A/F337A

Plasmid

#111787

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T336A/F337A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 Q340A/D341A

Plasmid

#111788

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q340A/D341A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

Plasmid

#74055

Purpose

retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)

Use

Retroviral

Tags

AVI-TEV and FLAG

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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hFLN29-Mid/pCMV-14-FLAG (L175-D390)

Plasmid

#73589

Purpose

Express middle part (L175-D390) of human TRAFD1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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