We narrowed to 4,340 results for: Pet2;

1. His10-PS-SNAPf-9R (XSB525)

   Plasmid

   #78513

   Purpose

   Construct for expressing His10 - SNAPf -9R in E. Coli

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   SNAPf
   

   Tags

   His10 followed by a prescission protease claevage…

   Expression

   Bacterial

   Mutation

   9R added to the C-terminal of SNAPf

   Available Since

   June 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK598

   Plasmid

   #72523

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK560

   Plasmid

   #71585

   Purpose

   Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE )
   

   Expression

   Bacterial

   Mutation

   changed arginine-232 to histidine

   Promoter

   T7 promoter

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK504

   Plasmid

   #71584

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-357 to tyrosine

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK612

   Plasmid

   #72533

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK613

   Plasmid

   #72534

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK619

   Plasmid

   #72536

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pfMDH_K381A

    Plasmid

    #67732

    Purpose

    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 381 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only