We narrowed to 8,779 results for: chloramphenicol

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pDGE655

Plasmid

#153253

Purpose

M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_024

Plasmid

#123686

Purpose

Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L2-C3 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_023

Plasmid

#123685

Purpose

Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1- B1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LC101: pMVP/PB/Neo-DEST

Plasmid

#121875

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBWB514

Plasmid

#209326

Purpose

p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MTK2_007

Plasmid

#123702

Purpose

Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pEF1a

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pC029 - Lw2Cas13a from Leptotrichia wadei F0279

Plasmid

#91919

Purpose

Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Lw2Cas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

Plasmid

#91918

Purpose

Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbfCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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MTK234_008

Plasmid

#123902

Purpose

Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GFP dropout for multiTU destination vectors

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4_003

Plasmid

#123812

Purpose

Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rglb pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42

Plasmid

#153525

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#42

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#42

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK301

Plasmid

#110593

Purpose

BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Bba_B0015 Terminator

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFNC-3

Plasmid

#233296

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Inserts

BxbI phage integrase
sacB

Expression

Bacterial

Promoter

PEM7 and unknown

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15

Plasmid

#153523

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#15

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#15

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEC3111 (pSpy1C-Plac(3)_ffluc)

Plasmid

#218778

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of mrfp cassette, mutagenesis of the RBS…

Promoter

Plac(3)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MSP2262

Plasmid

#70703

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A & H557A in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE476

Plasmid

#153267

Purpose

M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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