We narrowed to 4,719 results for: pcr

1. pFA6a-A1-FLAG:kanMX6

   Plasmid

   #134430

   Purpose

   PCR-based C-terminal A1-FLAG tag

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   A1-FLAG
   

   Use

   Pcr-based tagging in yeast

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pFA6a-A1-FLAG:HIS3MX6

   Plasmid

   #134431

   Purpose

   PCR-based C-terminal A1-FLAG tag

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   A1-FLAG
   

   Use

   Pcr-based tagging in yeast

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pFA6a-S6-FLAG:hphMX4

   Plasmid

   #134437

   Purpose

   PCR-based C-terminal S6-FLAG tag

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   S6-FLAG
   

   Use

   Pcr-based tagging in yeast

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pOliSpec5'

   Plasmid

   #228551

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOliSpec3'

   Plasmid

   #228550

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpec3'

   Plasmid

   #228548

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH136

   Plasmid

   #47554

   Purpose

   pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

   Insert

   URA3
   

   Use

   Pcr template

   Tags

   Expression

   Mutation

   Promoter

   Native URA3 promoter, -243 to -1.

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMaCTag-Z07

   Plasmid

   #124790

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMaCTag-Z13

    Plasmid

    #120056

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMaCTag-P21

    Plasmid

    #120032

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    24xSunTag
    

    Use

    Pcr template

    Tags

    24xSunTag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMaCTag-P20

    Plasmid

    #120031

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    10xSuntag
    

    Use

    Pcr template

    Tags

    10xSuntag

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaCTag-05

    Plasmid

    #119984

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMaCTag-Z15

    Plasmid

    #120058

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-Z08

    Plasmid

    #120051

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-Z12

    Plasmid

    #120055

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMaCTag-23

    Plasmid

    #120002

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P23

    Plasmid

    #120034

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-P05

    Plasmid

    #120016

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge