We narrowed to 59,446 results for: cloning

1. epi-BE4max

   Plasmid

   #135975

   Purpose

   Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

   Depositor

   Yongming Wang

   Article

   Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV/TO Puro DEST (670-1)

   Plasmid

   #17293

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

   Plasmid

   #185713

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed W403 and F753 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

   Plasmid

   #115516

   Purpose

   Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Promoter

   UAS

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQTEV- PRDX2

   Plasmid

   #31338

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   PRDX2 (PRDX2 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCP60 RS-GFP

   Plasmid

   #122173

   Purpose

   "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

   Depositor

   Lukas Fischer

   Article

   Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

   Insert

   RS-GFP
   

   Use

   Binary vector for escherichia coli and agrobacter…

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mouse Clock delta 19

    Plasmid

    #177312

    Purpose

    expression of mutant mouse clock

    Depositor

    Joseph Takahashi

    Article

    King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

    Insert

    mouse clock delta 19
    

    Expression

    Mammalian

    Mutation

    deletion from original mClock gene (514-564aa)

    Promoter

    CMV

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG_attP

    Plasmid

    #110095

    Purpose

    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-B/c

    Plasmid

    #227962

    Purpose

    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQTEV-PSMD11

    Plasmid

    #31333

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti_sgRNA(MS2)_zsGreen1

    Plasmid

    #138463

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    zsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO GFP HSPH1

    Plasmid

    #19480

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPH1 (HSPH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET YBX-1

    Plasmid

    #13035

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

    Insert

    YBX1 (YBX1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pPT-TAR C1

    Plasmid

    #141246

    Purpose

    Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Algal Research, 2020. 50, 102014

    Insert

    Mitochondrial Genome of Phaeodactylum tricornutum
    

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Mutation

    Minor additional mutations described in manuscript

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO GFP HSPA1A

    Plasmid

    #19483

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1A (HSPA1A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO V5 DNAJB2a

    Plasmid

    #19523

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a (DNAJB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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