We narrowed to 13,804 results for: gan

1. pJM160

   Plasmid

   #134169

   Purpose

   Bacterial expression of RB3 stathmin-like domain (SLD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   RB3-SLD (STMN4 Human)
   

   Expression

   Bacterial

   Mutation

   residues 49-189

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC_HA_PP7-mCherry

   Plasmid

   #107605

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mCherry
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   hUBC

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK0_003

   Plasmid

   #123925

   Purpose

   Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Cas9-sgRNA destination
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR_hEMSY

   Plasmid

   #125156

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   EMSY (EMSY Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHES941

   Plasmid

   #87942

   Purpose

   Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   GAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Budding Yeast, Human)
   

   Expression

   Yeast

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-pelB-LaG-21

   Plasmid

   #172749

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-21
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TetO-FUW-mir124a

   Plasmid

   #61539

   Purpose

   Expresses mir124a in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   mir124a (MIR124-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

9. DNA-CARzeta-IRESpuro

   Plasmid

   #89345

   Purpose

   2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   DNA-CARzeta
   

   Use

   Lentiviral

   Tags

   SNAPf tag

   Expression

   Mammalian

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Orai1

    Plasmid

    #12199

    Depositor

    Anjana Rao

    Article

    Feske et al Nature. 2006 May 11. 441(7090):179-85.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pTorPE-IP2.0

    Plasmid

    #109217

    Purpose

    Express IP2.0 in E. coli

    Depositor

    Takeshi Ishihara

    Article

    Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

    Insert

    IP2.0 (Inverse Pericam 2.0)
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKC158

    Plasmid

    #134242

    Purpose

    CENP-A Halo expression construct

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP-A-Halo tagging construct (CENPA Human)
    

    Use

    Human targeting

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6895 PHAGE-P CMVT N-HA-hE6AP I wt

    Plasmid

    #37601

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I wt (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV tet regulatable

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-V5-Smpd4

    Plasmid

    #120237

    Purpose

    Expresses V5-tagged Smpd4 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Smpd4 (Smpd4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_PIKFYVE_DEP

    Plasmid

    #109789

    Purpose

    Protein expression and purification of PIKFYVE_DEP

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PIKFYVE_DEP (PIKFYVE Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSTAP-seq_human-4xUAS

    Plasmid

    #125150

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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