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pWZL-hygro-FAKS910A

Plasmid

#216542

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation S910A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mClover3

Plasmid

#209034

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5Q-GS3

Plasmid

#204746

Purpose

Expression of Cas9 and human H2AX with the K5Q mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5Q (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5Q mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5R-GS3

Plasmid

#204747

Purpose

Expression of Cas9 and human H2AX with the K5R mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5R (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5R mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134L-GS3

Plasmid

#204750

Purpose

Expression of Cas9 and human H2AX with the K134L mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134L (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134L mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134M-GS3

Plasmid

#204751

Purpose

Expression of Cas9 and human H2AX with the K134M mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134M (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134M mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134A-GS3

Plasmid

#204752

Purpose

Expression of Cas9 and human H2AX with the K134A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134A mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT25

Plasmid

#218721

Purpose

Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT25 (HSPB11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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