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pCDNA4TO-3XFLAG Halo WT nsp1
Plasmid#175412PurposeExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 nsp1 (ORF1ab CoV2)
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 CoV-2 nsp1 3xFLAG
Plasmid#176057PurposeExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.DepositorInsertCoV-2 nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag Intersectin Long (human)
Plasmid#47393DepositorInsertintersectin long (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-HADH2
Plasmid#67863Purposebacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)DepositorInsertHADH2 (HSD17B10 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag Intersectin Short (human)
Plasmid#47392DepositorInsertIntersectin short (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo K164A/H165A nsp1
Plasmid#175421PurposeExpresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SHIP2 WT pBABE-His-Xpress
Plasmid#126584PurposeRetroviral infection of MEF cells (puromycin selection)DepositorInsertSHIP2 (INPPL1 Human)
UseRetroviralTagsHis Xpress (N-terminal)ExpressionMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-hNEUROG2-iRPT
Plasmid#140764PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2DepositorInsertsUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO R99A CoV-2 nsp1 3xFLAG
Plasmid#176061PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.DepositorInsertCoV-2 nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
MigR1 Fli-1 GFP
Plasmid#24668DepositorInsertFli-1 (Fli1 Mouse)
UseRetroviralTagsGFP and IRESExpressionMutationPromoterAvailable sinceMay 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo delta 1-117 nsp1
Plasmid#175423PurposeExpresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 1-117 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationnsp1 C-terminusPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo delta 122-130 nsp1
Plasmid#175425PurposeExpresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 122-130 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo delta 118-180 nsp1
Plasmid#175424PurposeExpresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 delta 118-180 nsp1
UseTags3XFLAG HaloExpressionMammalianMutationN-terminus of nsp1PromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R124A/K125A nsp1
Plasmid#175417PurposeExpresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R124A/K125A nsp1
UseTags3XFLAG HaloExpressionMammalianMutationR124A and K125APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_POU5F1_P2A_Hygro_Barcode
Plasmid#120474PurposeBarcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPOU5F1 (POU5F1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn2
Plasmid#132712PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn geneDepositorInsertshDyn(2) (Pdyn Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-myc-His
Plasmid#67864Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tagDepositorInsertMRPP3 (KIAA0391 Human)
UseTagsHis and mycExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHelA12
Plasmid#176493PurposeExpresses the N-terminal His6-tagged SARS-CoV helicase in E coli.DepositorInsertSARS-CoV NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationV570IPromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
